Rodgers (Rg) and Chido (Ch) are blood-group antigens and they determine the fourthcomponentof human complement C4.Rodgers and Chido are associated with two C4isotypes (C4A and C4B).In addition to genotype determination, study on Belt expression ofRg and Chido could be useful in disease studies.DNA was extracted from the whole blood of 60 normal individuals.
Then, PCR amplificationof C4d gene fragment The Slacker Jean was followed by restriction digestion.This studydemonstrated that the frequency of Ch and Rg in Iranian healthy population was98.3 and 93.4 percent, respectively.
Additionally, 6.6 percent of the studied populationshowed Chido-positive, Rodger-negative and 1.7 percent showed Rodger-positive,Chido-negative genotype.It may be concluded that upon receiving blood transfusion,6.
6 and 1.7 percent of individuals could produce anti-Rg and anti-Ch antibodies, respectively.